Bisphosphonate가 배양된 치주인대세포의 조골작용에 미치는 효과
Effect of bisphosphonate on osteoblastic activity of the human periodontal ligament cells in vitro
구강과학- 전남대학교 2001년 13권 1호 p.35 ~ 49
김은영, 김지영,
소속 상세정보
김은영 ( ) - 전남대학교 치과대학 치주과학교실
김지영 ( ) - 전남대학교 치과대학 치주과학교실
KMID : 0988420010130010035
Abstract
Previous studies have demonstrated an increase in bone mass density with use of
bisphosphonate in osteoporosis. This agent acts as an inhibitor of osteoclastic
activity and results in increase of net osteoblastic activity.
The purpose of the present study was to examine the effect of the bisphosphonate on osteoblastic activity of the human periodontal ligament cells in vitro. Periodontal ligament cells were primarily obtained from extracted healthy third molars. Cells of 4th to 6th passage were cultured in Dulbecco´s modified Eagle´s medium containing alendronate sodium or etidronate disodium at the concentration of 10^-12∼10^-16㏖/L in 5% CO_2 incubator at 37℃. Cell count and MTT assay for cellular activity were done at 2 to 7 days of culture. Alkaline phosphatase activity at 4 to 7 days of culture and formation of mineralized nodules at 28 days of culture with addition of 50㎍/㎖ ascorbic acid, 10mM β glycerophosphate, 10^-7M dexamethasone were evaluated.
1. Alendronate sodium
Compared to the control, the proliferation of periodontal ligament cells was generally increased and the cellular activity was maintained at 2 days of culture and generally decreased at 7 days of culture. Alkaline phosphatase activity of periodontal ligament cells was increased and the formation of mineralized nodules by periodontal ligament cells was enhanced compared to the control.
2. Etidronate disodium
The proliferation of periodontal ligament cells increased at 2 days of culture and decreased or maintained at 7 days of culture. Compared to the control, the cellular activity of periodontal ligament cells was generally decreased. Alkaline phosphatase activity of periodontal ligament cells was increased and the formation of mineralized nodules by periodontal ligament cells was enhanced compared to the control.
These results suggest that alendronate sodium and etidronate disodium may have a
potential effect on osteoblastic lineage of periodontal ligament cells, distinct from their inhibitory action on osteoclasts and could contribute to enhance periodontal regeneration and alveolar bone regeneration.
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